Plasmid

Part:BBa_I745015:Design

Designed by: Princeton iGEM2007   Group: iGEM07_Princeton   (2007-10-26)


pLV-UBC-miR661-GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2012
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 2006
    Illegal SpeI site found at 1175
    Illegal SpeI site found at 7329
    Illegal SpeI site found at 8003
    Illegal PstI site found at 2629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2012
    Illegal SpeI site found at 1175
    Illegal SpeI site found at 7329
    Illegal SpeI site found at 8003
    Illegal PstI site found at 2629
    Illegal NotI site found at 1998
    Illegal NotI site found at 8039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2012
    Illegal BglII site found at 2793
    Illegal BglII site found at 2859
    Illegal BglII site found at 2900
    Illegal BglII site found at 7092
    Illegal BglII site found at 8091
    Illegal BamHI site found at 1254
    Illegal XhoI site found at 1236
    Illegal XhoI site found at 8046
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2012
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 2006
    Illegal SpeI site found at 1175
    Illegal SpeI site found at 7329
    Illegal SpeI site found at 8003
    Illegal PstI site found at 2629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2012
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 2006
    Illegal SpeI site found at 1175
    Illegal SpeI site found at 7329
    Illegal SpeI site found at 8003
    Illegal PstI site found at 2629
    Illegal NgoMIV site found at 3488
    Illegal NgoMIV site found at 4521
    Illegal NgoMIV site found at 4582
    Illegal NgoMIV site found at 4696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2882
    Illegal BsaI site found at 8073
    Illegal BsaI.rc site found at 487
    Illegal BsaI.rc site found at 2642
    Illegal BsaI.rc site found at 6224
    Illegal BsaI.rc site found at 7962
    Illegal BsaI.rc site found at 8697
    Illegal SapI site found at 5141


Design Notes

Cloned into the BamHI site of the pFUGW plasmid upstream of the EGFP gene.


Source

Princeton iGEM2007 team.

References